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Establishment of a PPR Global Research and Expertise Network (PPR-GREN)

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From:
Paul Rossiter <[log in to unmask]>
Reply To:
Paul Rossiter <[log in to unmask]>
Date:
Fri, 14 Feb 2014 10:18:02 +0000
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Dear Moderator, 

In responds to William's question. 
 
The oculo-nasal swabs too were positive for PPR (RNA) even though at different percentage with the primers F1/F2 by Forsyth and Barrett, 1995 and different NP3/NP4 by Couacy-Hymann et al., 2002. Whereas both primers were able to pick the virus from buffy coat perhaps due to the fact that the virus is propagated in the lymphoid and epitheliod tissues that leads to leucopenia and immunosuppression . 
 
I will love to attach the article for your perusal.

Thank you



>
>Dear Moderator, 
>
>
>In responds to William's question. 
>The oculo-nasal swabs too were positive for PPR (RNA) even though at different percentage with the primers F1/F2 by Forsyth and Barrett, 1995 and different NP3/NP4 by Couacy-Hymann et al., 2002. Whereas both primers were able to pick the virus from buffy coat perhaps due to the fact that the virus is propagated in the lymphoid and epitheliod tissues that leads to leucopenia and immunosuppression . 
>I will love to attach the article for your perusal.
>
>
>Thank you
>
>
>
>
>
>On Fri, Feb 14, 2014 at 9:51 AM, Paul Rossiter <[log in to unmask]> wrote:
>
>Dear Moderator
>> 
>>I have a question regarding Pam Luka’s observation that all of the buffy coats were positive for PPR (RNA).
>> 
>>Question: were any of the oculo-nasal swabs positive for PPR RNA which would seem to be a more important result (if confirmed) as it would suggest the ability to transmit the virus between animals ?  This is very relevant. Moderator. 
>> 
>>Thank you 
>> 
>>Dr William DUNDON  | Consultant Molecular Microbiologist APHL|
>>Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture | 
>>Department of Nuclear Sciences and Applications |
>>International Atomic Energy Agency| IAEA Laboratories Seibersdorf |
>>Vienna International Centre, PO Box 100, 1400 Vienna, Austria |
>>Email: [log in to unmask] T: (+43-1) 2600-28379
>>Follow us on www.iaea.org
>>     
>>Information on Nuclear Sciences and Applications, visit www-naweb.iaea.org
>> 
>>
>>
>>
>>
>>----- Forwarded Message -----
>>>From: "[log in to unmask]" <[log in to unmask]>
>>>To: [log in to unmask] 
>>>Sent: Friday, 14 February 2014, 11:29
>>>Subject: RE: From Dr Pam Luka, Vom, NIgeria on seromonitoring and on random diagnosis: plus moderator's request
>>>
>>>
>>>
>>>Dear Moderator
>>> 
>>>I have a question regarding Pam Luka’s observation that all of the buffy coats were positive for PPR (RNA).
>>> 
>>>Question: were any of the oculo-nasal swabs positive for PPR RNA which would seem to be a more important result (if confirmed) as it would suggest the ability to transmit the virus between animals ?
>>> 
>>>Thank you 
>>> 
>>>Dr William DUNDON  | Consultant Molecular Microbiologist APHL|
>>>Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture | 
>>>Department of Nuclear Sciences and Applications |
>>>International Atomic Energy Agency| IAEA Laboratories Seibersdorf |
>>>Vienna International Centre, PO Box 100, 1400 Vienna, Austria |
>>>Email: [log in to unmask] T: (+43-1) 2600-28379
>>>Follow us on www.iaea.org
>>>     
>>>Information on Nuclear Sciences and Applications, visit www-naweb.iaea.org
>>> 
>>>you,
>>> 
>>> 
>>>From:Establishment of a PPR Global Research and Expertise Network (PPR-GREN) [mailto:[log in to unmask]] On Behalf Of [log in to unmask]
>>>Sent: Thursday, 13 February 2014 17:38
>>>To: [log in to unmask]
>>>Subject: From Dr Pam Luka, Vom, NIgeria on seromonitoring and on random diagnosis: plus moderator's request
>>>
>>>Dear Paul,
>>>
>>>My name is Pam Luka from the National Veterinary Research Institute,
>>>Vom Nigeria. I agreed with other colleagues on the need for post
>>>vaccination seromonitoring as an indicator of whether we are getting
>>>it right or not. The cost of that maybe much but the benefits are also
>>>going to be a kind of "post vaccination positive/negative control".
>>>
>>>After the reported PPR outbreak and in the Karamoja region, Uganda in
>>>2006 even thought Wanwayi et al.,1995 reported the the presence of
>>>antibodies from repository samples collected in the 1980s. Where did
>>>the virus go then? Perhaps it vanished into thin air or eventually
>>>found a niche for itself and compounded with poor disease surveillance
>>>and disease reporting it remained unreported.
>>>
>>>Vaccination was carried out after the outbreak and we went back and
>>>carried out post-vaccination seromonitoring in 2008-2009 and discovered
>>>seropositivity of 55.3 %  for both sheep and goats. This was not so
>>>good for achieving "herd immunity" but it informed us of the need to
>>>conduct a second round of vaccination.
>>>
>>>During the same survey, we also randomly collected oculo-nasal
>>>swabs and buffy coat from apparently healthy animals and all the buffy
>>>coats turned out positive by reverse transcriptase PCR. 
>>>I assume you mean positive for PPR virus. 
>>>Are there some participants who could comment on this result please - Moderator .
>>>So if we are to go after the virus, we should consider collecting what kind of
>>>samples to collected in other not to miss it.
>>>
>>>
>>>Best regards
>>>
>>>Luka D. Pam
>>>Applied Mol. Biol. Div.
>>>NVRI, Vom.
>>>Plateau State,
>>>Nigeria
>>>
>>>
>>>
>>>To unsubscribe from the FAO-AnimalHealth-L list, click the following link:
>>>https://listserv.fao.org/cgi-bin/wa?SUBED1=FAO-AnimalHealth-L&A=1 
>>>This email message is intended only for the use of the named recipient. Information contained in this email message and its attachments may be privileged, confidential and protected from disclosure. If you are not the intended recipient, please do not read, copy, use or disclose this communication to others. Also please notify the sender by replying to this message and then delete it from your system. 
>>>
>>>
>>
>>To unsubscribe from the FAO-AnimalHealth-L list, click the following link:
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>
>
>
>-- 
>Luka D. Pam
>Applied Mol. Biol. Div.
>NVRI, Vom.
>Plateau State,
>Nigeria
>
>
>
>
>
>
>
>
>

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