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Establishment of a PPR Global Research and Expertise Network (PPR-GREN)

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From:
Paul Rossiter <[log in to unmask]>
Reply To:
Paul Rossiter <[log in to unmask]>
Date:
Tue, 11 Feb 2014 08:48:09 +0000
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A challenge for those of you with a molecular bent - Moderator.
 
Dear All,
As far as I know there is no multiplex Real-time RT-qPCR which detects PPR and PPR-like diseases (as individual agent or  co-infections) in a single tube.
Therefore I think further scientific researches must include a multiplex real time (RT)-qPCR which can detect PPR, BT, BDV, pox,contagious ecthyma and FMD viruses (and Beta-Actin internal control to check extraction and also PCR mix) in a single tube  (or double).
In current technology because generally Real-time PCR machines let us detect 5 different dyes, it is theoretically possible to detect 4 diseases and 1 internal control RNA/DNA in a single tube. However I am not sure it will be ever possible to do this in a single tube because of cross reactions. 
 

With my Best Wishes

M.Fatih BARUT D.V.M PhD 
Virological Diagnosis Lab.
Etlik Central Veterinary Control and Research Institute 
Kecioren / ANKARA /TURKEY 06020

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