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Establishment of a PPR Global Research and Expertise Network (PPR-GREN)

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From:
Paul Rossiter <[log in to unmask]>
Reply To:
Paul Rossiter <[log in to unmask]>
Date:
Mon, 24 Feb 2014 07:52:40 +0000
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Many thanks to Dr Aamer Zahur for this reply to a question from the moderator following  Dr Aamers's contribution on prolonged PPR virus excretion in the faeces of recovering small ruminants (Thursday 13th Feb). Please also see the related contribution from Dr Michael Baron (Friday 14th Feb). 
 
Dear Paul,
 
Sorry for delay in replying your inquiry because i was out of town. we detected PPR viral RNA through real-time RT-PCR however we were not be able to isolate PPR virus from the faeces of recovered animals despite many attempts. Definitely we will conduct an experiment to ascertain that weather these recovered animals transmit PPR virus to in contact naive animals or not however, our field observations are suggestive that such un-noticed transmissions may occurs.    

Best Regards 
Dr. Aamer Bin Zahur
DVM, M.Sc(Hons), PhD (Vet.Virology)
Principal Scientific Officer/Program Leader/Associate Professor  
Animal Health Laboratories, ASI, NARC
Park Road, Islamabad. Pakistan
Ph : +92 51 9255029, +92 51 8443916 
Fax: +92 51 9255420
Cell:+92 300 9502369



On Thursday, February 13, 2014 5:32 Dr Zahur wrote:

 
Dear All

I am Aamer Bin Zahur working on the epidemiology, diagnosis and progressive control of PPR in Pakistan. first of all i  must say that the e-conference idea has facilitated  interesting exchange of many experiences and findings regarding PPR control. I want to share observations with all the colleagues. 

It was observed that several outbreaks initiated after the introduction of new animals in to the flock however, those newly introduced animals remained unaffected during the entire course of outbreak. Furthermore it was also observed that some of the outbreaks occurred two to three weeks after the introduction of animals in to the flock. therefore a longitudinal study was conducted to ascertain the role of recovered animals in the epidemiology of PPR. it was found that many of the recovered animals persistently at least up to sixteenth week post outbreak. however, when vaccination was conducted at the phase of the outbreak to entire flock i.e both affected and unaffected animals, the virus shedding was not observed in recovered animals. 

So this evidence may be kept in mind while developing a control program for PPR
 
Best Regards shed PPR virus in their fecal material 
 
Dr. Aamer Bin Zahur
DVM, M.Sc(Hons), PhD 
Principal Scientific Officer/Program Leader/Associate Professor 
Animal Health Research Laboratories, ASI, NARC
Park Road, Islamabad. Pakistan
Ph : +92 51 9255029, +92 51 8443916 
Fax: +92 51 9255420
Cell:+92 300 9502369
 
 
 
 
 
Dear Dr Aamer, I am sure many readers would like to know how you detected the virus in the faeces of the recovered animals- was it live virus recovered in cell-culture or virus antigen or RNA?  Have you been able to carry out  experiments to see if you can transmit infection to susceptible stock kept in-contact with these "recovered" animals? And what  species were these animals?  Kind regards, Moderator. 

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