Dear All,
Just want to mention something. I am little bit against vaccination and instead support to increase individual cell mediated specific immunity with the help of Transfer Factor and neutralizing monoclonal antibody cocktail
transfusions.
As a little reminder, in our situation,Transfer Factors are PPR specific T and B- Lymphocytes extracts and their interleukins which we produce in-vivo or in-vitro. And monoclonal antibodies for cocktail are Anti-F (which is against fusion protein of the virus
and inhibits the virus to enter cell) and Anti-H (which is against hemagglutinin protein of the virus and inhibits virus
to hold erythrocytes therefore inhibits virus spread to body via blood).
Let us say we have a well informed, conscious field veterinarian who can distinguish PPR with %90 accuracy with just symptoms without (before) laboratory confirmation. And who can act immediately and quarantine the herd to
prevent
sick animals to contact other herds.Then he demands Anti-PPR Transfer factor and Monoclonal antibody
cocktails from an institution -a laboratory- or from a company. And with the help of intravenous injection of Anti-PPR immunisation cocktail he increases the herd immunity immediately to the desired amount. We catch the virus immediately and have eliminated
it in the herd we detected.
On the other hand if we use vaccination we have to consider
the problems below;
1- Pestivirus contamination. I detected Pestivirus several times in very well known companies' foetal calf serums. And companies and institutes are using those serums for cell cultured vaccines. That means a little lack of attention can cause really big iatrogenic
problems. In my opinion high prevalence of BDV and also BVDV is because of pestivirus contaminated vaccines.
2- Contamination of other viruses and bacterias, it is also reported problem.
3- Not sufficient inactivation of the virus or mutation of the vaccine virus in field.
4- Producing million doses of vaccines, storing it, Hiring staff and vehicles for vaccinations and also the weight and milk production losses because of the after vaccination stress really increase the budget.
5- Persuading animal owner for vaccination is not always possible also some farms are not reachable because of the geographical and/or climate difficulties that means some animals will be unvaccinated. However if there is PPR in a herd it is very easy to convince
the animal owner for treatment (via the way explained above), and if there is PPR in a herd even
animal owner can take you there with his own vehicle.
6-Especially in sheep herds, vaccination is done by automatic vaccination guns. Guns have a tank or use vaccination bottle itself. The bottle can be transparent for sunlight and that means 1 hour of sunlight exposure for vaccination virus.
To sum up, in my opinion vaccination can be urgent solution for current situation but in the future at least for our researches we need to focus on the solutions which includes immediate specific immune system boosters.
I asked Dr Fatih for some estimates of cost for this novel approach and he kindly provided the following - Moderator
"I think much less than $100,000 is enough to establish a lab which is able to:
1- Catch 2 hybridoma clones (Hybridoma technology) for continuous production of anti-F protein and anti-H protein monoclonal antibodies. (includes experimental animals/animal units)
2- Maintain those hybridomas (special incubators medias and so on) and harvest their monoclonals, freeze and keep them until use.
3- Establish a T and B lymphocyte cell culture lab. (Hard part)
4- Produce Transfer Factor.
5- Combine Transfer Factor with Monoclonal antibody cocktail.
6- Add adjuvants and probably vitamins(natural immune boosters and antioxidants) and non specific antimicrobials for long shelf life.
7 Prepare the last product dispense it in vials and keep it safe until use".
Wish best luck with your studies.
Fatih Barut
Veterinarain
---
With my Best Wishes
M.Fatih BARUT D.V.M PhD
Virological Diagnosis Lab.
Etlik Central Veterinary Control and Research Institute
Kecioren / ANKARA /TURKEY 06020