Dear All, Yes I mean PPR virus detection by RT-PCR on buffy coats Regards >Dear All, > >Yes I mean PPR virus detection by RT-PCR on buffy coats > >Regards > > >On 2/13/14, Paul Rossiter <[log in to unmask]> wrote: >> Dear Paul, >> >> My name is Pam Luka from the National Veterinary Research Institute, >> Vom Nigeria. I agreed with other colleagues on the need for post >> vaccination seromonitoring as an indicator of whether we are getting >> it right or not. The cost of that maybe much but the benefits are also >> going to be a kind of "post vaccination positive/negative control". >> >> After the reported PPR outbreak and in the Karamoja region, Uganda in >> 2006 even thought Wanwayi et al.,1995 reported the the presence of >> antibodies from repository samples collected in the 1980s. Where did >> the virus go then? Perhaps it vanished into thin air or eventually >> found a niche for itself and compounded with poor disease surveillance >> and disease reporting it remained unreported. >> >> Vaccination was carried out after the outbreak and we went back and >> carried out post-vaccination seromonitoring in 2008-2009 and discovered >> seropositivity of 55.3 % for both sheep and goats. This was not so >> good for achieving "herd immunity" but it informed us of the need to >> conduct a second round of vaccination. >> >> During the same survey, we also randomly collected oculo-nasal >> swabs and buffy coat from apparently healthy animals and all the buffy >> coats turned out positive by reverse transcriptase PCR. >> I assume you mean positive for PPR virus. >> Are there some participants who could comment on this result please - >> Moderator . >> So if we are to go after the virus, we should consider collecting what kind >> of >> samples to collected in other not to miss it. >> >> >> Best regards >> >> Luka D. Pam >> Applied Mol. Biol. Div. >> NVRI, Vom. >> Plateau State, >> Nigeria >> >> ######################################################################## >> >> To unsubscribe from the FAO-AnimalHealth-L list, click the following link: >> https://listserv.fao.org/cgi-bin/wa?SUBED1=FAO-AnimalHealth-L&A=1 > > >-- >Luka D. Pam >Applied Mol. Biol. Div. >NVRI, Vom. >Plateau State, >Nigeria > > > ######################################################################## To unsubscribe from the FAO-AnimalHealth-L list, click the following link: https://listserv.fao.org/cgi-bin/wa?SUBED1=FAO-AnimalHealth-L&A=1