Dear All,

Yes I mean PPR virus detection by RT-PCR on buffy coats

Regards


 
 
 
 
 


>Dear All,
>
>Yes I mean PPR virus detection by RT-PCR on buffy coats
>
>Regards
>
>
>On 2/13/14, Paul Rossiter <[log in to unmask]> wrote:
>> Dear Paul,
>>
>> My name is Pam Luka from the National Veterinary Research Institute,
>> Vom Nigeria. I agreed with other colleagues on the need for post
>> vaccination seromonitoring as an indicator of whether we are getting
>> it right or not. The cost of that maybe much but the benefits are also
>> going to be a kind of "post vaccination positive/negative control".
>>
>> After the reported PPR outbreak and in the Karamoja region, Uganda in
>> 2006 even thought Wanwayi et al.,1995 reported the the presence of
>> antibodies from repository samples collected in the 1980s. Where did
>> the virus go then? Perhaps it vanished into thin air or eventually
>> found a niche for itself and compounded with poor disease surveillance
>> and disease reporting it remained unreported.
>>
>> Vaccination was carried out after the outbreak and we went back and
>> carried out post-vaccination seromonitoring in 2008-2009 and discovered
>> seropositivity of 55.3 %  for both sheep and goats. This was not so
>> good for achieving "herd immunity" but it informed us of the need to
>> conduct a second round of vaccination.
>>
>> During the same survey, we also randomly collected oculo-nasal
>> swabs and buffy coat from apparently healthy animals and all the buffy
>> coats turned out positive by reverse transcriptase PCR.
>> I assume you mean positive for PPR virus.
>> Are there some participants who could comment on this result please -
>> Moderator .
>> So if we are to go after the virus, we should consider collecting what kind
>> of
>> samples to collected in other not to miss it.
>>
>>
>> Best regards
>>
>> Luka D. Pam
>> Applied Mol. Biol. Div.
>> NVRI, Vom.
>> Plateau State,
>> Nigeria
>>
>> ########################################################################
>>
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>
>
>-- 
>Luka D. Pam
>Applied Mol. Biol. Div.
>NVRI, Vom.
>Plateau State,
>Nigeria
>
>
>

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