Dear All,
Yes I mean PPR virus detection by RT-PCR on buffy coats
Regards
On 2/13/14, Paul Rossiter <
[log in to unmask]> wrote:
> Dear Paul,
>
> My name is Pam Luka from the National Veterinary Research Institute,
> Vom Nigeria. I agreed with other colleagues on the need for post
> vaccination seromonitoring as an indicator of whether we are getting
> it right or not. The cost of that maybe much but the benefits are also
> going to be a kind of "post vaccination positive/negative control".
>
> After the reported PPR outbreak and in the Karamoja region, Uganda in
> 2006 even thought Wanwayi et al.,1995 reported the the presence of
> antibodies from repository samples collected in the 1980s. Where did
>
the virus go then? Perhaps it vanished into thin air or eventually
> found a niche for itself and compounded with poor disease surveillance
> and disease reporting it remained unreported.
>
> Vaccination was carried out after the outbreak and we went back and
> carried out post-vaccination seromonitoring in 2008-2009 and discovered
> seropositivity of 55.3 % for both sheep and goats. This was not so
> good for achieving "herd immunity" but it informed us of the need to
> conduct a second round of vaccination.
>
> During the same survey, we also randomly collected oculo-nasal
> swabs and buffy coat from apparently healthy animals and all the buffy
> coats turned out positive by reverse transcriptase PCR.
> I assume you mean positive for PPR virus.
> Are there some participants who could comment on this result please -
> Moderator .
> So if we are to go
after the virus, we should consider collecting what kind
> of
> samples to collected in other not to miss it.
>
>
> Best regards
>
> Luka D. Pam
> Applied Mol. Biol. Div.
> NVRI, Vom.
> Plateau State,
> Nigeria
>
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Luka D. Pam
Applied Mol. Biol. Div.
NVRI, Vom.
Plateau State,
Nigeria