Thanks Dr. Paul.The PPRV (PPRV/Nanakpur/2012) I talked about was isolated in beginning of 2012 from a farmers goatherd, a few months after I started my job at the Goat Institute where I am currently working. Indeed it was my first outbreak but the the affected animals exhibited all the symptoms of PPR, like those described in the text book (Tongue lesions, nasal and ocular discharge, diarrhoea, pneumonia). My senior colleagues who went with me as well as myself were pretty much convinced that it was due to PPRV.The farmers had about 125 animals (mostly sheep/goats as well as 4 cattle), about 70 of which were died (cattle were healthy) by the time we reached there. Six were died in the preceding night and we also performed the postmortem and collected the specimens. The farmer purchased the animals from 3-4 different places and he had no idea about the vaccination history against PPR. Some of the animals (old) what he had been were claimed to be vaccinated a year ago (though I am not sure about his claim). The farmer usually don't have tagging system so its difficult to trace the history. We provided the supportive treatment (antibiotics/antipyretics/antidiarrhoeal/vitamins) but did not vaccinate them. Later we came to know that about 20 more animals died. Under such conditions in India usually the farmer used to sale the animals may be at lower price just to get the last minute benefit. We could not visit that farm again but this may be the case with that farmer as well. This is why in absence of restricted animal movement its difficult to contain the disease in India scenario.If I conclude, there was no definite history of the vaccination against PPR in that farm, therefore at this moment it would not appropriate to say that the PPRV under study evade the vaccination (Sungri96). However, the in vitro studies carried out by me with Mabs and hyperimmune as well as the antigen capture ELISA (based on the reagents from Indian vaccine strain (Sungri96)) suggest that yes this virus may evade the immune response generated against the vaccine strain.One more thing I would like to clear which is not mentioned in the manuscript (as it was performed later) is that the hyperimmune serum from Indian vaccine strain (PPRV/Sungri/96) and PPRV/Nanakpur/2012 gave an SNT titer of 4-8 and 64-128 respectively while neutralising PPRV/Nanakpur/2012. Its being neutralised by vaccine serum but a poor reactivity as compared to homologous serum.The whole genome sequencing of this new PPR virus is under pipeline, I hope that we would able to do it soon.Thanks and regardsNaveen Kumar, BVSc&AH, MVSc,PhDSenior Scientist (Veterinary Virology)Room No.118, Division of Animal HealthCentral Institute for Research on GoatsIndian Council of Agricultural ResearchMakhdoom, PO-Farah-281122District-Mathura, UPIndia+91 08171301889 (cell)Fax:+91 565-2763246email: [log in to unmask]web: http://www.cirg.res.in/goat_health.php
To unsubscribe from the FAO-AnimalHealth-L list, click the following link:
https://listserv.fao.org/cgi-bin/wa?SUBED1=FAO-AnimalHealth-L&A=1
--
Naveen Kumar, BVSc&AH, MVSc,PhDSenior Scientist (Veterinary Virology)Room No.118, Division of Animal HealthCentral Institute for Research on GoatsIndian Council of Agricultural ResearchMakhdoom, PO-Farah-281122District-Mathura, UPIndiaTel: +91 565-2763260 Ext 269+91 08171301889 (cell)Fax:+91 565-2763246email: [log in to unmask]web: http://www.cirg.res.in/goat_health.php
To unsubscribe from the FAO-AnimalHealth-L list, click the following link:
https://listserv.fao.org/cgi-bin/wa?SUBED1=FAO-AnimalHealth-L&A=1